3NA4
D53P beta-2 microglobulin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 29.070, 50.730, 71.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.220 - 1.900 |
| R-factor | 0.21702 |
| Rwork | 0.211 |
| R-free | 0.26865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z9t |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.533 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.910 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.106 | 0.569 |
| Number of reflections | 8702 | |
| <I/σ(I)> | 8.4 | 2.4 |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 4000, Sodium Acetate, Glycerol, Ammonium Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
