3N89
KH domains
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.504, 102.241, 83.608 |
| Unit cell angles | 90.00, 119.32, 90.00 |
Refinement procedure
| Resolution | 48.113 - 2.789 |
| Rwork | 0.259 |
| R-free | 0.27600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.214 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 3.070 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Number of reflections | 28664 | |
| <I/σ(I)> | 8.61 | 2.3 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Ammoniumsulfate, Hepes, Glycerol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Ammoniumsulfate, Hepes, Glycerol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
