3N7Q

Crystal structure of human mitochondrial mTERF fragment (aa 99-399) in complex with a 12-mer DNA encompassing the tRNALeu(UUR) binding sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-05
Spacegroup name	P 32
Unit cell lengths	99.851, 99.851, 39.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.000 - 2.400
R-factor	0.2306
Rwork	0.228
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.342
Data reduction software	XDS
Data scaling software	SHELX
Phasing software	EPMR
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	2.550
High resolution limit [Å]	2.400	2.400
Number of reflections	17262

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION			VAPOR DIFFUSION