3N7C
Crystal structure of the RAN binding domain from the nuclear pore complex component NUP2 from Ashbya gossypii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-10 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.540, 62.550, 76.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.170 - 2.260 |
| R-factor | 0.236 |
| Rwork | 0.234 |
| R-free | 0.29300 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.774 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (C) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.120 | 2.380 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 13396 | |
| <I/σ(I)> | 15.7 | 5.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 13.5 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 100mM Bis-Tris pH 5.5 + 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
