3N6V
Structure of the GluA2 NTD-dimer interface mutant, T78A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2010-04-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9395 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.210, 120.150, 360.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.776 - 3.200 |
| R-factor | 0.237 |
| Rwork | 0.235 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hsy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.226 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.776 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.635 | |
| Total number of observations | 49741 | |
| Number of reflections | 47323 | |
| <I/σ(I)> | 10.9 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 100mM Tris, 200mM MgCl2, 20% PEG 8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
