3N5N
Crystal structure analysis of the catalytic domain and interdomain connector of human MutY homologue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.65 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.311, 82.167, 63.460 |
| Unit cell angles | 90.00, 100.90, 90.00 |
Refinement procedure
| Resolution | 62.310 - 2.300 |
| R-factor | 0.20843 |
| Rwork | 0.206 |
| R-free | 0.25078 |
| Structure solution method | SAD and Molecular Replacement |
| Starting model (for MR) | 1muy |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.991 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE (plus Molrep) |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.310 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 25872 | |
| <I/σ(I)> | 18.7 | 4.6 |
| Completeness [%] | 95.8 | 93.4 |
| Redundancy | 7.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 298 | 20% PEG 3350, 0.2M Magnesium acetate, 0.005 M tris[2-carboxyethyl] phosphine (TCEP), 5% glycerol, 0.01 M spermidine, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
