3N4Z
Crystal structure of quintuple Arg-to-Lys variant of T. celer L30e
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2009-01-20 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 30.487, 61.360, 102.767 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.489 - 2.397 |
R-factor | 0.1887 |
Rwork | 0.182 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h7m |
RMSD bond length | 0.007 |
RMSD bond angle | 1.069 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.440 | 2.530 |
High resolution limit [Å] | 2.397 | 2.397 |
Rmerge | 0.126 | 0.268 |
Total number of observations | 119186 | |
Number of reflections | 8086 | |
<I/σ(I)> | 15.4 | 8.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.7 | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.8M potassium phosphate, 0.8M sodium phosphate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.8M potassium phosphate, 0.8M sodium phosphate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |