3N4M
E. coli RNA polymerase alpha subunit C-terminal domain in complex with CAP and DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.10 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 175.730, 175.730, 160.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.510 | 3.180 |
High resolution limit [Å] | 2.987 | 3.020 |
Rmerge | 0.085 | 1.106 |
Number of reflections | 29113 | |
<I/σ(I)> | 11.5 | 1.6 |
Completeness [%] | 98.9 | 99.5 |
Redundancy | 4.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293.2 | 100 mM sodium acetate (pH 4.5), 625 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K |