3N42
Crystal structures of the mature envelope glycoprotein complex (furin cleavage) of Chikungunya virus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-11-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.391, 90.678, 178.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.600 - 3.000 |
| R-factor | 0.249 |
| Rwork | 0.247 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | P62E1 ENVELOPE GLYCOPROTEINS FROM CHIKUNGUNYA VIRUS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 18964 | |
| <I/σ(I)> | 9.2 | 2 |
| Completeness [%] | 96.4 | 82.2 |
| Redundancy | 3.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 8-12% PEG4K, 100mM NaAcetate, 100mM Hepes pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
