3N3W
2.2 Angstrom Resolution Crystal Structure of Nuclease Domain of Ribonuclase III (rnc) from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97965 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.099, 98.252, 118.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 2.205 |
| R-factor | 0.17922 |
| Rwork | 0.176 |
| R-free | 0.23371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a11 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.118 | 0.513 |
| Number of reflections | 18659 | |
| <I/σ(I)> | 16.7 | 3.3 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 7.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | Protein solution: 7.6 mg/mL, 0.5M Sodium chloride, 0.01M Tris pH 8.3 Screen solution: Classics II (F7), 0.2M Ammonium sulfate, 0.1M BIS-TRIS pH 6.5, 25% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
