3N3E

Zebrafish AlphaA crystallin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	33.297, 33.297, 354.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.615 - 1.750
R-factor	0.207
Rwork	0.205
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l1e
RMSD bond length	0.007
RMSD bond angle	1.108
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.810
High resolution limit [Å]	1.750	3.770	1.750
Rmerge	0.063	0.041	0.344
Number of reflections	21407
<I/σ(I)>	11.4
Completeness [%]	98.2	90.9	99.6
Redundancy	7	5.7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.1M MIB pH 6.0, 25% PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 298K