3N39

Ribonucleotide Reductase Dimanganese(II)-NrdF from Escherichia coli in Complex with NrdI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	113
Detector technology	CCD
Collection date	2009-12-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0781
Spacegroup name	P 21 21 21
Unit cell lengths	75.403, 90.721, 143.787
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.840 - 2.500
R-factor	0.237
Rwork	0.235
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3N37 and 1RLJ
RMSD bond length	0.006
RMSD bond angle	0.856
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.840
High resolution limit [Å]	2.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.6		20% PEG 3000, 0.1 M HEPES pH 7.6, 0.1 M lithium sulfate