3N32
The crystal structure of human Ubiquitin adduct with Zeise's salt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.071 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.060, 40.450, 49.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.094 - 1.795 |
| R-factor | 0.1841 |
| Rwork | 0.181 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.093 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | ILMILIONE (v.2.2) |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.390 | 1.890 |
| High resolution limit [Å] | 1.795 | 1.795 |
| Rmerge | 0.040 | 0.198 |
| Number of reflections | 5572 | |
| <I/σ(I)> | 27.4 | 7 |
| Completeness [%] | 95.6 | 85.6 |
| Redundancy | 4.4 | 4.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 30% PEG 1450, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
