3N2Z
The Structure of Human Prolylcarboxypeptidase at 2.80 Angstroms Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | H 3 2 |
Unit cell lengths | 181.140, 181.140, 240.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.670 - 2.790 |
R-factor | 0.218 |
Rwork | 0.216 |
R-free | 0.24400 |
Structure solution method | MIRAS |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.790 | 2.790 |
Rmerge | 0.098 | 0.594 |
Number of reflections | 37814 | |
<I/σ(I)> | 9.9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.9 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.8M Ammonium Sulfate, 0.1M HEPES, 1-2% PEG400 mixed in a 2:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |