3N2Y
Crystal structure of tyrosyl-tRNA synthetase complexed with p-(2-tetrazolyl)-phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-21 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 43 |
| Unit cell lengths | 101.115, 101.115, 71.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.220 - 2.490 |
| R-factor | 0.244 |
| Rwork | 0.242 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j1u |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.128 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.486 | 2.486 |
| Rmerge | 0.084 | 0.332 |
| Number of reflections | 24812 | |
| <I/σ(I)> | 19.8 | 2.7 |
| Completeness [%] | 97.1 | 77 |
| Redundancy | 6.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 283 | 100mM Tris , 4% PEG 8000, 16% PEG 300, 10% glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
