3N2A
Crystal structure of bifunctional folylpolyglutamate synthase/dihydrofolate synthase from Yersinia pestis CO92
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.853, 78.791, 124.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.17736 |
| Rwork | 0.176 |
| R-free | 0.20238 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w7k |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.447 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 0.610 |
| Number of reflections | 48106 | |
| <I/σ(I)> | 33.7 | 3.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.5 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M Magnesium formate 20% Peg 3350 15 % glycerol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
