3N1B

C-terminal domain of Vps54 subunit of the GARP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.700
Spacegroup name	C 1 2 1
Unit cell lengths	125.862, 30.323, 88.953
Unit cell angles	90.00, 120.50, 90.00

Refinement procedure

Resolution	30.976 - 2.398
R-factor	0.241
Rwork	0.239
R-free	0.28700
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	RESOLVE (2.13)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.398	5.170	2.400
Rmerge	0.066	0.061	0.200
Number of reflections	11565
<I/σ(I)>	18.4
Completeness [%]	98.4	98.6	90.3
Redundancy	3.3	3.3	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	23% PEG3350, 0.1M BisTris, 0.1M I3C, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	23% PEG3350, 0.1M BisTris, 0.1M I3C, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K