3N0X
Crystal structure of an ABC-type branched-chain amino acid transporter (RPA4397) from Rhodopseudomonas palustris CGA009 at 1.50 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-10 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97949,0.97898 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.736, 41.806, 75.979 |
Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
Resolution | 29.348 - 1.500 |
R-factor | 0.149 |
Rwork | 0.148 |
R-free | 0.17200 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.508 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.348 | 29.348 | 1.550 |
High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
Rmerge | 0.043 | 0.018 | 0.441 |
Number of reflections | 55240 | 10494 | 9643 |
<I/σ(I)> | 11.69 | 36.2 | 1.7 |
Completeness [%] | 96.6 | 96.5 | 94.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2000M NaOAc, 30.0000% PEG-8000, 0.1M Cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |