3N0P
A mutant human Prolactin receptor antagonist H30A in complex with the extracellular domain of the human prolactin receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0810 |
Spacegroup name | P 65 |
Unit cell lengths | 123.540, 123.540, 73.330 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.670 - 2.100 |
R-factor | 0.18223 |
Rwork | 0.180 |
R-free | 0.22388 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mzg |
RMSD bond length | 0.025 |
RMSD bond angle | 2.049 |
Data reduction software | MOSFLM (3.3.9) |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 106.989 |
High resolution limit [Å] | 1.787 |
Number of reflections | 37230 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 3.7M NaCl, 0.1 M Hepes pH 7.5, temperature 291K, VAPOR DIFFUSION, HANGING DROP |