3N0C
TM0449 mutant crystal grown by hanging drop method
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.549, 116.718, 141.202 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.19585 |
Rwork | 0.193 |
R-free | 0.24754 |
Structure solution method | Rigid body refinemet |
Starting model (for MR) | 3nob |
RMSD bond length | 0.018 |
RMSD bond angle | 1.832 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.089 | 0.520 |
Number of reflections | 40698 | |
<I/σ(I)> | 12.8 | 2.7 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 295 | 49% PEG 200, 0.1M HEPES (pH 7.6), VAPOR DIFFUSION, HANGING DROP, temperature 295K |