3N06
A mutant human Prolactin receptor antagonist H27A in complex with the extracellular domain of the human prolactin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.419, 123.419, 72.665 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.857 - 2.000 |
| R-factor | 0.19704 |
| Rwork | 0.195 |
| R-free | 0.24051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mzg |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.000 | 31.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.131 | 0.063 | 0.940 |
| Number of reflections | 42787 | ||
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 17.5 | 21.7 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 3.6M NaCl, 0.1 M Hepes 7.5, temperature 291K, VAPOR DIFFUSION, HANGING DROP |
