3MZI
Crystallographic structure of the pseudo-Signaling State of the BLUF Photoreceptor PixD (slr1694) Y8F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.240 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.850, 119.480, 93.260 |
| Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
| Resolution | 49.925 - 2.304 |
| R-factor | 0.1665 |
| Rwork | 0.165 |
| R-free | 0.21550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2HFN; chain A |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.241 |
| Data reduction software | XDS |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.217 |
| Number of reflections | 37424 | |
| <I/σ(I)> | 13.08 | 4.49 |
| Completeness [%] | 53.9 | 8 |
| Redundancy | 3.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.1 M (NH4)2SO4, 0.1 M Bis-Tris, 0.01 M Spermidine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
