3MYX

Crystal structure of a PSPTO_0244 (Protein with unknown function which belongs to Pfam DUF861 family) from Pseudomonas syringae pv. tomato str. DC3000 at 1.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97913,0.97879
Spacegroup name	P 1 21 1
Unit cell lengths	57.584, 48.396, 80.454
Unit cell angles	90.00, 108.21, 90.00

Refinement procedure

Resolution	28.759 - 1.300
R-factor	0.119
Rwork	0.117
R-free	0.14600
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.495
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.759		1.350
High resolution limit [Å]	1.300	2.800	1.300
Rmerge	0.042	0.019	0.308
Number of reflections	101752	19843	17364
<I/σ(I)>	12.07	33.6	2.4
Completeness [%]	96.4	97.4	80.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	277	0.2000M NH4Cl, 20.0000% PEG-3350, No Buffer pH 6.3, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K