3MYK
Insights into the Importance of Hydrogen Bonding in the Gamma-Phosphate Binding Pocket of Myosin: Structural and Functional Studies of Ser236
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97885 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.005, 147.021, 153.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.910 - 1.840 |
| R-factor | 0.19458 |
| Rwork | 0.193 |
| R-free | 0.23009 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vom |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.045 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.910 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.051 | 0.152 |
| Number of reflections | 84770 | |
| <I/σ(I)> | 35.7 | 5.3 |
| Completeness [%] | 98.7 | 97.6 |
| Redundancy | 6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 12% PEG8K, 250mM MgCl2, 100mM MOPS, 2mM AMPPNP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
