3MXN
Crystal structure of the RMI core complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Detector technology | CCD |
| Collection date | 2009-07-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97933, 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.096, 42.223, 152.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.800 - 1.550 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.23106 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.191 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 39281 | |
| <I/σ(I)> | 25.7 | 2.4 |
| Completeness [%] | 97.0 | 83 |
| Redundancy | 10.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 10 mM Hepes pH 7.1, 0.05 M NaSCN, 20% ethylene glycol, 2 mM DTT, 13% polyethylene glycol 3350, with benzamidine (1%) added to the drop, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
