3MXI
TREX1 3' Exonuclease D18N Familial Chilblain Lupus Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.028, 58.776, 66.523 |
| Unit cell angles | 90.00, 110.33, 90.00 |
Refinement procedure
| Resolution | 31.476 - 2.550 |
| R-factor | 0.2121 |
| Rwork | 0.209 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.280 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.4_113)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.780 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.100 | 0.230 |
| Number of reflections | 14777 | |
| <I/σ(I)> | 10.9 | 4.5 |
| Completeness [%] | 96.6 | 95.1 |
| Redundancy | 6.9 | 6.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 0.15 M MES pH 6.5 19% PEG 4000 10% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
