3MX9
Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 72.170, 170.190, 46.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.286 - 2.600 |
| R-factor | 0.1843 |
| Rwork | 0.181 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.224 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.330 | 2.410 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.058 | 0.011 |
| Number of reflections | 26884 | |
| <I/σ(I)> | 8.3 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 20% PEG 400, 0.1M MES pH 6.5, 5% ethanol, 10% ethylene glycol, VAPOR DIFFUSION, temperature 298K |
