3MX8
Crystal structure of ribonuclease A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.607, 32.667, 73.188 |
| Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
| Resolution | 29.142 - 2.100 |
| R-factor | 0.183 |
| Rwork | 0.179 |
| R-free | 0.25800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3mwq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.990 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.151 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.109 | 0.577 |
| Total number of observations | 8347 | |
| Number of reflections | 14239 | |
| <I/σ(I)> | 5.397 | 1.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 286 | 30% PEG 8000, 0.2M ammonium sulfate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
