3MX7
Crystal Structure Analysis of Human FAIM-NTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-16 |
| Detector | SBC-2 |
| Wavelength(s) | 1.04361 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.170, 57.170, 69.946 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.570 - 1.760 |
| R-factor | 0.17759 |
| Rwork | 0.176 |
| R-free | 0.20045 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.741 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.069 | 0.187 |
| Number of reflections | 14391 | |
| <I/σ(I)> | 17.267 | 11.041 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 5.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.67M sodium phosphate, 0.73M potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
