3MWY
Crystal structure of the chromodomain-ATPase portion of the yeast Chd1 chromatin remodeler
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792,0.9611 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 94.333, 94.333, 450.088 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.830 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.069 | 0.402 |
Number of reflections | 20114 | |
<I/σ(I)> | 20.9 | 4.5 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 18% PEG 3350; 400 mM K+/Na+ tartrate; 5% xylitol; 10 mM MgCl2; 1 mM ATPgammaS, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |