3MWR
Crystal structure of ribonuclease A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-07-07 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.903, 33.067, 73.869 |
Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
Resolution | 24.033 - 1.850 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mwq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.808 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.21) |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 73.922 | 24.630 | 1.950 |
High resolution limit [Å] | 1.850 | 5.850 | 1.850 |
Rmerge | 0.080 | 0.055 | 0.558 |
Total number of observations | 3422 | 14884 | |
Number of reflections | 21141 | ||
<I/σ(I)> | 5.804 | 10 | 1.3 |
Completeness [%] | 98.6 | 94.1 | 97.3 |
Redundancy | 5 | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 286 | 30% PEG 8000, 0.2M ammonium sulfate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 286K |