3MWQ

Crystal structure of ribonuclease A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-07-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	101.632, 32.915, 73.391
Unit cell angles	90.00, 91.03, 90.00

Refinement procedure

Resolution	24.460 - 1.680
R-factor	0.176
Rwork	0.174
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1srn
RMSD bond length	0.004
RMSD bond angle	0.909
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.21)
Phasing software	AMoRE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.379	24.460	1.740
High resolution limit [Å]	1.650	5.220	1.650
Rmerge	0.055	0.049	0.282
Total number of observations		4392	7772
Number of reflections	27506
<I/σ(I)>	5.949	9.6	2.7
Completeness [%]	92.6	99.2	57.4
Redundancy	4.3	4.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	286	30% PEG 8000, 0.2M ammonium sulfate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 286K