3MWL
Q28E mutant of HERA N-terminal RecA-like domain in complex with 8-OXOADENOSINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.178, 92.178, 108.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.906 - 1.600 |
| R-factor | 0.1976 |
| Rwork | 0.196 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gxs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.107 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.906 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.028 | 0.590 |
| Number of reflections | 60859 | |
| <I/σ(I)> | 13.2 | 1.1 |
| Completeness [%] | 97.3 | 70.1 |
| Redundancy | 8.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |
