3MWK
Q28E mutant of HERA N-terminal RecA-like domain, complex with 8-oxo-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-04-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.916, 91.916, 108.006 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.960 - 1.450 |
| R-factor | 0.16105 |
| Rwork | 0.159 |
| R-free | 0.20914 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gxs |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.981 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.019 | 0.596 |
| Number of reflections | 82310 | |
| <I/σ(I)> | 15.7 | 1.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 15.2 | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |
