3MWE
Truncated Human ATP-Citrate Lyase with Tartrate Bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.191, 60.793, 109.176 |
| Unit cell angles | 90.00, 124.96, 90.00 |
Refinement procedure
| Resolution | 44.736 - 2.200 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.22750 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.089 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.640 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.079 | 0.450 |
| Number of reflections | 45944 | |
| <I/σ(I)> | 9.2 | 2.4 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 294 | 10% (w/v) PEG 3350, 100 mM sodium tartrate, 100 mM Tris-HCl, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
