3MW6
Crystal structure of NMB1681 from Neisseria meningitidis MC58, a FinO-like RNA chaperone
Replaces: 2HXJExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.479, 76.527, 122.715 |
| Unit cell angles | 90.00, 116.84, 90.00 |
Refinement procedure
| Resolution | 42.889 - 2.209 |
| R-factor | 0.1929 |
| Rwork | 0.191 |
| R-free | 0.23480 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.088 | 0.464 |
| Number of reflections | 96019 | |
| <I/σ(I)> | 26.2 | 1.92 |
| Completeness [%] | 97.1 | 79.2 |
| Redundancy | 4.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 100MM BIS-TRIS, 170MM MGCL2, 21% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
