3MVT
Crystal structure of apo mADA at 2.2A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.833, 93.827, 86.180 |
| Unit cell angles | 90.00, 96.76, 90.00 |
Refinement procedure
| Resolution | 33.900 - 2.200 |
| R-factor | 0.18973 |
| Rwork | 0.187 |
| R-free | 0.24494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a4m |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.207 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (from CCP4) |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.093 | 0.369 |
| Number of reflections | 37983 | |
| <I/σ(I)> | 10.7 | 2.5 |
| Completeness [%] | 95.7 | 89.9 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 295 | 200 mM Ammonium sulfate, 20 % PEG 3350, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
