3MVD
Crystal structure of the chromatin factor RCC1 in complex with the nucleosome core particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.681, 183.036, 107.030 |
| Unit cell angles | 90.00, 101.52, 90.00 |
Refinement procedure
| Resolution | 34.701 - 2.900 |
| R-factor | 0.1759 |
| Rwork | 0.175 |
| R-free | 0.21550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kx5 and Drosophila RCC1(not yet deposited in PDB) |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.626 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER ((CCP4-6.1.1)) |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.058 | 0.590 |
| Number of reflections | 85040 | |
| <I/σ(I)> | 22.25 | 1.67 |
| Completeness [%] | 98.5 | |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 25 mM sodium acetate buffer, 25 mM sodium citrate, 1 mM DTT, 6 % PEG2000-MME, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium acetate | ||
| 2 | 1 | 1 | sodium citrate | ||
| 3 | 1 | 1 | DTT | ||
| 4 | 1 | 1 | PEG2000-MME | ||
| 5 | 1 | 2 | sodium acetate | ||
| 6 | 1 | 2 | sodium citrate | ||
| 7 | 1 | 2 | DTT | ||
| 8 | 1 | 2 | PEG2000-MME |
