3MV3
Crystal Structure of a-COP in Complex with e-COP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97960 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 328.126, 74.310, 96.396 |
| Unit cell angles | 90.00, 101.98, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.250 |
| Rwork | 0.241 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.630 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.370 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 62887 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 100 | |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 100 mM HEPES, pH 7.2 8 % (w/v) tacsimate 100 mM sodium malonate 200 mM sodium chloride 20 % (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
