3MV1
E.Coli (lacZ) beta-galactosidase (R599A) in complex with Guanidinium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 151.730, 161.520, 203.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.290 - 2.200 |
| Rwork | 0.205 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dp0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.290 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.104 | 0.470 |
| Number of reflections | 251768 | |
| <I/σ(I)> | 7.7 | 3 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 3.06 | 3.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | PEG8000, NaCl, MgCl2, DTT, Bis-Tris, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
