3MUY
E. coli (lacZ) beta-galactosidase (R599A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.11587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 149.266, 167.237, 200.614 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 86.020 - 2.500 |
Rwork | 0.179 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dp0 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.020 | 2.320 |
High resolution limit [Å] | 2.500 | 2.200 |
Rmerge | 0.183 | 0.851 |
Number of reflections | 253030 | |
<I/σ(I)> | 6.7 | 1.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.26 | 4.32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | PEG 8000, NaCl, MgCl2, DTT, Bis-Tris, pH 6.5, HANGING DROP AT 288K, VAPOR DIFFUSION, HANGING DROP |