3MUJ
Early B-cell factor 3 (EBF3) IPT/TIG and dimerization helices
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-26 |
| Detector | RAYONIX MX-225 |
| Spacegroup name | P 65 |
| Unit cell lengths | 134.680, 134.680, 41.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.100 - 1.920 |
| R-factor | 0.171 |
| Rwork | 0.170 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mqi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.476 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.100 | 1.960 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.057 | 0.473 |
| Number of reflections | 32303 | |
| <I/σ(I)> | 30.82 | 4.64 |
| Completeness [%] | 94.0 | 17.3 |
| Redundancy | 9.1 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 277 | 0.1M BTP, 0.2M Naformate, 21% PEG3350, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
