3MUD
Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-06 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 96.564, 96.564, 162.993 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.20913 |
| Rwork | 0.207 |
| R-free | 0.25049 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fu1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.016 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.057 | |
| Number of reflections | 39647 | |
| <I/σ(I)> | 49.6 | 5.8 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 18.6 | 17.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18% MePEG 2000, 100 mM Bis-Tris, 120 mM MgSO4 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
