3MTI
The Crystal Structure of a rRNA Methylase from Streptococcus thermophilus to 1.95A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.331, 57.044, 75.365 |
| Unit cell angles | 90.00, 109.11, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.27200 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
| Rmerge | 0.085 | 0.066 | 0.578 |
| Number of reflections | 28265 | ||
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 99.5 | 96.9 | 99.9 |
| Redundancy | 3.3 | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 25% PEG 3350, 0.1M HEPES pH 7.5, 0.2M Sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
