3MR0
Crystal Structure of Sensory Box Histidine Kinase/Response Regulator from Burkholderia thailandensis E264
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-04 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.265, 35.080, 71.348 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 30.578 - 1.493 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.309 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.578 | 1.540 |
| High resolution limit [Å] | 1.493 | 1.500 |
| Number of reflections | 46689 | |
| <I/σ(I)> | 17.7 | 2.4 |
| Completeness [%] | 97.2 | 75.6 |
| Redundancy | 4.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Lithium sulfate, 0.1 M TRIS pH 8.5, 40 %v/v PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
