3MQW
Crystal structure of a putative endoribonuclease L-PSP from Entamoeba histolytica with higher solvent content and an ordered N-terminal tag
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-08 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.000, 99.720, 106.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.860 - 1.800 |
R-factor | 0.148 |
Rwork | 0.147 |
R-free | 0.18300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3m4s |
RMSD bond length | 0.019 |
RMSD bond angle | 1.518 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.860 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.074 | 0.424 |
Number of reflections | 81926 | |
<I/σ(I)> | 14.29 | 2.7 |
Completeness [%] | 98.6 | 90.9 |
Redundancy | 4.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 290 | JCSG+ SCREEN CONDITION F2: 100MM CITRATE, 3150 MM AMMONIUM SULFATE, ENHIA.00579.A AT 26 MG/ML IN THE PRESENCE OF 2 MM SODIUM ORTHOVANADATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |