3MQS

Crystal Structure of the USP7:Hdm2(PSTS) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 41
Unit cell lengths	69.840, 69.840, 45.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.800 - 2.400
R-factor	0.214
Rwork	0.214
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yy6
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	CNS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.800	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	8727
<I/σ(I)>	5.9	2.6
Redundancy	4.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	30% PEG4000, 0.2M Lithium Sulfate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K