3MQI
Human early B-cell factor 1 (EBF1) IPT/TIG domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-02 |
| Wavelength(s) | 1.00764 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.291, 57.073, 69.140 |
| Unit cell angles | 90.00, 93.17, 90.00 |
Refinement procedure
| Resolution | 28.530 - 2.300 |
| R-factor | 0.23532 |
| Rwork | 0.233 |
| R-free | 0.27275 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.151 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.530 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.095 | 0.726 |
| Number of reflections | 14710 | |
| <I/σ(I)> | 16.78 | 2.72 |
| Completeness [%] | 13.5 | 13.24 |
| Redundancy | 7.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 0.1M Tris, 23% PEG MME, 0.3M trimethylamine n-oxide, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
