3MQB
Crystal Structure of Ectodomain of BST-2/Tetherin/CD317 (C2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0717 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.650, 28.590, 94.510 |
| Unit cell angles | 90.00, 111.43, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.25832 |
| Rwork | 0.255 |
| R-free | 0.31526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mq7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.081 | 0.569 |
| Number of reflections | 7240 | |
| <I/σ(I)> | 12.5 | 1.9 |
| Completeness [%] | 93.8 | 96.7 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 298 | Crystals were grown at 25C using the microbatch under-oil method by mixing protein with crystallization buffer containing 100 mM HEPES, pH 7.5, 30% PEG 4000. 3-6% DMSO and 0.2-0.5 mM TCEP were used as additives for optimal crystal growth., EVAPORATION, temperature 298.0K |
