3MPY
Structure of EUTM in 2-D protein membrane
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 6 |
| Unit cell lengths | 69.164, 69.164, 28.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.290 - 2.000 |
| R-factor | 0.22641 |
| Rwork | 0.224 |
| R-free | 0.28277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2A1B (RESIDUES 2-90) |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.459 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 17.300 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 4477 |
| <I/σ(I)> | 20.1 |
| Completeness [%] | 81.1 |
| Redundancy | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2 M ammoniumsulfate, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
